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KMID : 0881720210360020118
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Journal of Food Hygiene and Safety
2021 Volume.36 No. 2 p.118 ~ p.123
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In vivo Genotoxicity Assessment of Matrine and the Water Extract of Sophorae Radix Using a Comet Assay
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Heo Seong-Moo
Lee Ji-Yeon
Jeon Hyoung-Woon
Kim Myoung-Jun
Chung Young-Shin
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Abstract
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The genotoxic potentials of the water extract of Sophorae Radix (WSR) and matrine as a majorcompound were investigated in murine hepatocytes and blood cells using single-cell gel electrophoresis. According tothe test guidelines of OECD TG 489, an in vivo alkaline Comet assay was performed using male mice. The treatmentdoses of the WSR were 1,000 and 2,000 mg/kg body weight to include the limiting dose. The three test doses formatrine were 50, 100, and 200 mg/kg, and the maximum dose was estimated from a dose range-finding test. The negativecontrol and test articles were administered three times by oral gavage, and the positive control (ethyl methanesulfonate,EMS) was administered twice. Within 3~4 hours after the last treatment, the liver tissue and blood werecollected, and single-cell electrophoresis was performed under alkaline conditions. The in vivo Comet assay showedno changes in body weights during the treatments with the test articles. The WSR and matrine did not induce significantDNA damage in the liver and blood cells of mice compared to the vehicle treatment. In conclusion, matrine andthe WSR may not have genotoxic potential.
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KEYWORD
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Sophorae Radix, Matrine, in vivo Genotoxicity, Comet assay
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